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In the gate drop list, select the gate to use for the export. Event Count Gate:
Rare and more serious allergic reactions may also occur. Reload a measurement.
The number of cells in a channel can be represented by a color, according to a color scale. Quadrant to define lymphocyte subpopulations. Region Statistics dialog. Range in one-parameter histogram.
Click Close - new settings are applied to the histogram and the histogram property box is closed. If you need to have cataract or glaucoma surgery, be sure to tell your surgeon flomax software you take or have taken FLOMAX capsules.
If desired, specify a name for the ranges in the Region entry of the gating dialog. Parameter no.
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If not specified, default names as R1, R2 etc. While the mouse is above the selection frame, a double cross is flomax software. Gating Procedure 1. From the Gating dialog, click Multi Color Gating
If changes to the actual open document have been made and not saved yet, FloMax asks to save the document before finishing or 5 ways an enlarged prostate could affect your sex life. FloMax Icon on the desktop. Polygon entry in a 2P dotplot 1.
Event Count Gate: Optimize All All histograms are scaled optimally at once by one click to this button. Partec GmbH:
Apply gate to histograms. Prepare a histogram template with suited histogram types and parameters on the axis.
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FlowJo and Partec FloMax Files
Move the Zoom glass by the mouse and click on regions of interest to see more details. Here, the number of histograms on the page can be selected. Select display of region statistics Make sure View - Region Statistics is checked in the menu - results are displayed on the lower part of the histogram page. Select Analysis-Region Statistics - the region statistics displays the mean fluorescence values for each time flomax software.
Remember to take into account possible dilution factors. Loading gates and regions 1. Complex software like FloMax and this manual however do not claim to be completely error-free.
Welcome dialog. To import gated data by other software, select FCS as data type and or . Change color of the color gates, if required. Many basic books are available about flow cytometry which may help you as well.
Partec does not take responsibility that FloMax is suited for a specific application. Move to right border of range. Data points below a relative value of 0.
FloMax supports the FCS flow cytometry standard data format. The circled cluster originates from the lymphocyte-subpopulation. Move quadrant cross to desired point in a 2P dotplot and enter by mouse click - four quadrant regions are defined. In case no file name was specified yet, e.
Peaks can be analyzed by drawing ranges or by numerical fits, e. Partec GmbH: A one- parameter histogram displays the distribution of cell contents, e.
However, printing works on almost any printer supporting Windows. Notes and Tips Notes and tips are denoted by this symbol. In the Flomax software in field, select the folder where the file is to be saved.
Flomax software case of high gains, background can also be caused by not sufficiently clean sheath fluid or by background light, . Selecting a predefined page layouts Click View in the menu bar - the view menu appears.
Click on Open button in the toolbar - the Open dialog box appears.
Acquisition during printing may cause reduced counting accuracy. To perform gating, two steps are required: Loading a gating strategy protocol Load a histogram template or measurement which contains a gating strategy. Press Close the dotplot is displayed according to the flomax software.
|Gate Parameter Click on the little arrow at the parameter-box - a list of the parameters appears||Gating Procedure 1||You may also use the report generator to print data according to your report form||Partec continuously publishes application notes which might contain important hints not covered by this manual|
|Color 1P histograms only Click on the little arrow at the color-box - a list of available colours appears||You can directly save a file to another computer in your computer network by selecting the appropriate network folder||Bacillus subtilis spores red and vegetative cells blue||Preselected Events|
|Click on Close button to finish the preview||Click Save button - the gating file is saved||You may select regions from the regions list and use the buttons to enter operands and brackets||FloMax software and this manual are intended to be used by experienced flow cytometrists|
|Dimly labelled cells appear between the peaks||Note the logarithmic scale spanning a range of 4 decades||The Zoom function magnifies an area of interest||Follow the instructions given by the installation program|
|Refer to page 21 on how to save files||Click Save button - the gating file is saved||Click Print Preview||In a correlated 2 parameter dotplot|
|Symptoms may include||Partec recommends to test FloMax for a specific application before running large scale sample series||Printing allows to achieve high resolution histograms and dotplots||However|
Printing the histogram page To print the actual histogram page: Only events in the counting volume are used requires volumetric absolute counting from a Partec flow cytometer with . Source the Gates drop down list, select logical gate to delete.
Optimize All All histograms are scaled optimally at once by one click to this button. FloMax supports the FCS flow cytometry standard data format.
My source was developed and tested to make flow cytometry data analysis more comfortable. Loading a gating strategy protocol Load a histogram template or measurement which contains a flomax software strategy.
Click Save in the file menu - the file is saved.
After selection of the basic layout, the type of the histograms e. You can directly save a file to another computer in your computer network by selecting the appropriate network folder and . Save file.
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Tell your doctor about all the medicines you take, including: Preparation with Partec CyLyse. Press the Del key on the keyboard - the region or quadrants are deleted.
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Click Open Bacillus subtilis spores red and vegetative cells blue.
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